A medium compostion for lymphocyte derived from peripheral blood mononuclear cells having anticancer activity and cultivation method using the same

摘要

The present invention relates to a culture medium for the culture of anti-cancer activated lymphocytes derived from peripheral blood mononuclear cells, and a method for culturing cancer-activated lymphocytes using the same. More particularly, the present invention relates to a culture medium containing anti-CD3 monoclonal antibody , And anti-CD16 monoclonal antibody was included in the culture medium, the effect of promoting differentiation and mass proliferation of NK cells, NKT cells, and T cells (anti-cancer activated lymphocytes) was confirmed from peripheral blood mononuclear cells. Accordingly, the present invention relates to a culture composition based on the fractionated normal hexane fraction and a method for culturing peripheral blood mononuclear cells using the same. Also, the anti-cancer activated lymphocytes derived from peripheral blood mononuclear cells cultured by the method according to the present invention can be used as a pharmaceutical composition highly effective for cancer treatment. Furthermore, the culture medium composition of the present invention can be used as an anticancer therapy itself, and when a chemical anticancer agent is administered concurrently in a clinical trial, the dose of the conventional anticancer drug can be lowered, and the side effect can be alleviated and the anticancer activity can be greatly improved.