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Methods of cultivating plantlets in vitro derived from moringa roots

机译:辣木根离体培养的方法

摘要

The present invention relates to a method of forming plantlets of moringa in vitro, which comprises the steps of: (a) excising a fixed size of tissues from a root region of moringa (including growing points); and (b) in order to grow the excised tissues of the root region of moringa into plantlets, growing the excised tissues on a growth medium prepared by adding to a basal medium 30-40 g/L of sucrose, 30 g/L of D-sorbitol, 0.1 g/L of inositol, and 8-10 g/L of agar. According to the present invention, by enabling an early supply of a large quantity of moringa to farms, the method allows the farms to harvest leaves, stems, and roots of moringa even in limited climatic conditions in Korea, and thus enables the production of functional food materials containing moringa as a main ingredient. In addition, the method makes it possible to culture moringa in a convenient and efficient manner by directly using the tissues of moringa being cultured in vitro in improved culture conditions, and thus can shorten the moringa plantlet formation period to 8 weeks, and can enable mass propagation culture. In addition, the method can avoid losses due to poor germination rates in seedlings grown from imported seeds in conventional farms, and thus can contribute to increased economic gain in the farms, replacement of imported moringa, and expansion of moringa growing farms.
机译:本发明涉及一种在体外形成辣木幼苗的方法,该方法包括以下步骤:(a)从辣木的根部区域(包括生长点)切下一定大小的组织。 (b)为了将辣木的根部区域的切除的组织生长成小植株,将切除的组织在通过向基础培养基中添加30-40 g / L的蔗糖,30 g / L的D而制备的生长培养基上生长。 -山梨糖醇,0.1 g / L肌醇和8-10 g / L琼脂。根据本发明,通过使得能够尽早向农场供应大量的辣木,该方法允许农场即使在韩国的有限气候条件下也可以收获辣木的叶,茎和根,从而能够生产功能性的辣木。以辣木为主要成分的食品。另外,该方法使得可以通过直接使用在改良的培养条件下体外培养的辣木组织以方便而有效的方式来培养辣木,从而可以将辣木的幼苗形成期缩短至8周,并且可以使团聚。繁殖文化。另外,该方法可以避免由于在常规农场中从进口种子种植的幼苗中发芽率差而造成的损失,因此可以有助于增加农场的经济收益,替代进口的辣木和扩大辣木种植农场。

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