2 - A method for target protein identification using thermal stability shift-based fluorescence difference in two-dimensional gel electrophoresis

摘要

The present invention relates to an identifying method for a target protein using a thermal stability shift-based fluorescence difference in two-dimensional gel electrophoresis (TS-FITGE) and, more specifically, to a method for identifying a protein that is to be a target of a specific drug by analyzing thermal stability shifts in the protein, which occur when a specific drug or, desirably, a bioactive molecule is combined with a target protein, through FITGE. Therefore, the identifying method for a target protein using TS-FITGE can easily find a target protein even without labelling both a bioactive natural substance having an extreme chemical structure, that is, a natural substance having a complex structure difficult to be synthesized, and a small and simple natural substance having an insufficiently small space for chemical modification, by using a probe, and can be very efficient in developing an innovative new drug and treatment. In addition, the identifying method for a target protein using TS-FITGE can easily detect thermal stability shifts in a protein through noticeable color changes at a specific temperature even when changes in melting temperature due to thermal stability shifts in the protein are subtle or the slope of a melting profile is sharp, by combining the existing thermal stability assay with FITGE technology.