2 - A method for target protein identification using thermal stability shift-based fluorescence difference in two-dimensional gel electrophoresis

摘要

The present invention relates to a method for identifying a target protein using a thermostability-based fluorescence difference in two-dimensional gel electrophoresis, and more particularly, to a method for identifying a target protein using a specific drug, preferably a physiologically active molecule, The present invention relates to a method for identifying a target protein of a specific drug by analyzing a change in thermal stability of the protein through fluorescence difference of two-dimensional gel electrophoresis. When a target protein identification method using TS-FITGE according to the present invention is used, a physiologically active natural material having an extreme chemical structure, that is, a natural material having a complicated structure difficult to synthesize, and a small and simple natural material lacking space for chemical transformation, The target protein can be easily identified without labeling it, which is very efficient in the development of innovative new drugs and therapies. In addition, the existing thermal stability assays are combined with FITGE technology, so that even when the change of the fusion temperature due to the change of the thermal stability of the protein is small or the slope of the melting curve is small, It is easy to detect changes in thermal stability.