2 Method of simultaneous analysing for apamin melittin and phospholipase A2 in bee venom using Ultra-high performance liquid chromatography

摘要

The present invention relates to a method for simultaneous analysis of bee venom melittin, apamin and phospholipase A2 using ultrahigh performance liquid chromatography, and more particularly, to a method for simultaneous analysis of bee venom aqueous solution samples by means of ultra high performance liquid chromatography, , Apaminin and phospholipase A2 at the same time. Conventionally, only a single substance such as melitin has been identified as an index substance of bee venom and the result of the experiment on confirmation of the quality of bee venom was not accurate. However, in order to analyze the accurate content of bee venom through the present invention, By confirming how to analyze melittin, apamin and phospholipase A2 at the same time, beekeeping farmers and related companies can easily provide a way to reduce the costs associated with bee venom quality control and bee venom weight analysis .;