METHOD OF SEPARATING FUKINOLIC ACID FROM PETASITES JAPONICUS

摘要

The present invention relates to a method for separating fukinolic acid from a Petasites japonicus extract. According to the present invention, a large amount of high purity fukinolic acid can be obtained from the Petasites japonicus extract, and fukinolic acid can be used as a commercialized standard form. The method comprises the steps of: (a) adding water to Petasites japonicus leaves and performing extraction; (b) freeze-drying the extract; (c) adding ethanol to the extract and performing precipitation; (d) removing supernatant and freeze-drying the precipitate; (d) performing diaion column chromatography; (f) performing sephadex column chromatography; and (e) performing lichroprep column chromatography.