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GLUCOSE DEHYDROGENASE HAVING MODIFIED ELECTRON TRANSFER PROPERTIES, AND GLUCOSE MEASUREMENT METHOD
GLUCOSE DEHYDROGENASE HAVING MODIFIED ELECTRON TRANSFER PROPERTIES, AND GLUCOSE MEASUREMENT METHOD
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机译:具有修饰的电子转移性质的葡萄糖脱氢酶及其测量方法
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摘要
A glucose dehydrogenase having modified electron transfer properties, and a glucose measurement method and measuring kit using the glucose dehydrogenase are provided. Provided are a glucose dehydrogenase having at least 1, 2 or 3 amino acid substitutions, for example, amino acid substitution with a polar amino acid or alanine, in the region corresponding to the 457th to 477th position in SEQ ID NO: 1 of a glucose dehydrogenase having homology with SEQ ID NO: 1, a glucose measurement method, a measurement reagent kit and a sensor using the glucose dehydrogenase. The electron transfer properties of the glucose dehydrogenase of the present invention is modified and can be used in measuring glucose in the presence of a mediator with reduced concentration or in the absence of a mediator, and can be used, for example, in continuous glucose measurement.
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机译:提供了具有改变的电子传递性质的葡萄糖脱氢酶,以及使用该葡萄糖脱氢酶的葡萄糖测定方法和测定试剂盒。提供了一种葡萄糖脱氢酶,其在对应于葡萄糖的SEQ ID NO:1的第457至477位的区域中具有至少1、2或3个氨基酸取代,例如被极性氨基酸或丙氨酸取代的氨基酸。与SEQ ID NO:1具有同源性的脱氢酶,葡萄糖测量方法,测量试剂盒和使用该葡萄糖脱氢酶的传感器。本发明的葡萄糖脱氢酶的电子传递特性被修饰,可以在浓度降低的介体存在下或不存在介体的情况下用于葡萄糖的测定,例如可以用于连续葡萄糖测定中。 。
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