Glucose dehydrogenase and glucose measurement method with modified electron transfer properties

摘要

A glucose dehydrogenase with modified electron transfer characteristics, and a glucose measurement method and measurement kit using the glucose dehydrogenase are provided. Glucose dehydrogenase having homology to SEQ ID NO: 1 with at least one, two or three amino acid substitutions in the region corresponding to positions 457 to 477 in SEQ ID NO: 1, for example amino acid substitutions with polar amino acids or alanine And a glucose measuring method, a measuring reagent kit and a sensor using the same. The glucose dehydrogenase of the present invention has a modified electron transfer property and can perform glucose measurement in the presence of a lower concentration of mediator or in the absence of a mediator. For example, it can be used for continuous glucose measurement.