DETECTION METHOD OF CIRCULATING TUMOR CELLS AND PRETREATMENT METHOD FOR DETECTING CIRCULATING TUMOR CELLS

摘要

The present invention provides a method for detecting circulating tumor cells, comprising steps of: capturing cells in a blood sample on a filter; contacting an antibody that is labeled with a first fluorescent dye and recognizes a leukocyte marker protein with the cell-captured filter; contacting an animal serum, a surfactant and an antibody that is labeled with a second fluorescent dye and recognizes an epithelial cell marker protein with the cell-captured filter; contacting a third fluorescent dye that stains nucleic acids with the cell-captured filter; and detecting fluorescences emitted from each cell captured on the filter.