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USE OF A NITROGEN-DOPED POROUS CARBON MATERIAL FOR ENRICHING PHOSPHORYLATED PROTEINS OR PEPTIDES
USE OF A NITROGEN-DOPED POROUS CARBON MATERIAL FOR ENRICHING PHOSPHORYLATED PROTEINS OR PEPTIDES
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机译:掺氮多孔碳材料用于富集磷酸化蛋白质或肽的用途
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摘要
The present invention pertains to the use of a nitrogen-doped porous carbon material for the enrichment of phosphorylated peptides and/or proteins. The advantage of using a carbon-derived material is that it provides high surface area, high permeability containing both mesopores and macropores. Furthermore it retains polar and non-polar compounds and is extremely chemically stable. The key advantage of using a nitrogen-doped porous carbon material is the fact that it can be positively charged at low pH. This charge is crucial for the enrichment of phosphorylated proteins and peptides. The nitrogen-doped porous carbon material enriches all types of phosphorylated peptides; single, double, triple and quadruple phosphorylated. To our best knowledge this is the first material capable of achieving such a feat.
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