Generation of mature lineage from induced pluripotent stem cells with disrupted MeCP2

摘要

Provided herein are efficient in vitro maintenance, expansion, culture, and / or various erythroid, myeloid, lymphoid, or endoderm of pluripotent cells in which the MeCP2 gene is disrupted It is a method of differentiation into lineages, especially mature erythrocytes. Pluripotent cells can be maintained and differentiated under defined conditions. Thus, in certain embodiments, the use of mouse feeder cells or serum is not required for progenitor cell differentiation.