LGR5+ SOMATIC STEM CELLS

摘要

PROBLEM TO BE SOLVED: To provide a method of preparing an adult somatic stem cell and a population of the adult somatic stem cells that are Lgr5+ and can be easily isolated and maintained.;SOLUTION: A method of preparing an isolated somatic stem cell and a population of somatic stem cells from a blood or a bone marrow sample is provided, the somatic stem cell being 2 to less than 6 micrometers in size and being Lgr5+, CD9-, and CD349-. The method comprises the steps of incubating the sample with EDTA or heparin in a container until the blood or the bone marrow sample obtained from a subject is separated into an upper layer and a lower layer, collecting the upper layer, and isolating from the upper layer a population of somatic stem cells that are 2 to less than 6 micrometers in size and Lgr5+, CD9-, and CD349-.;SELECTED DRAWING: None;COPYRIGHT: (C)2019,JPO&INPIT