METHOD FOR CONCENTRATING HIGHLY FUNCTIONAL DIFFERENTIATION-INDUCED CELLS AND HIGHLY FUNCTIONAL DIFFERENTIATION-INDUCED CELL POPULATION

摘要

PROBLEM TO BE SOLVED: To provide methods for selecting and concentrating highly functional differentiation-induced cells from a pluripotent stem cell-derived differentiation-induced cell group, and additionally to provide a highly functional differentiation-induced cell population concentrated by the concentration method.;SOLUTION: The present invention provides a method by introducing a drug resistance gene into the gene locus of drug metabolizing enzyme CYP of pluripotent stem cells. Highly functional differentiation-induced cells, specifically, CYP high expression differentiation-induced hepatocytes and CYP high expression differentiation-induced small intestinal epithelial cells can be selected and concentrated by subjecting pluripotent stem cells in which the drug resistance gene is induced to differentiation-inducing treatment and by culturing obtained differentiation-induced cells in the presence of the drug corresponding to the drug resistance. It was confirmed that a highly functional differentiation-induced hepatocyte population obtained by concentration expresses CYP3A4, CYP1A2, CYP2C19, and the like which are markers of mature hepatocytes, equally to human primary culture hepatocytes, and a highly functional differentiation-induced small intestinal epithelial cell population expresses Villin, ISX, ANPEP, and the like which are small intestinal epithelial cell markers, equally to human small intestinal cells.;SELECTED DRAWING: Figure 21;COPYRIGHT: (C)2019,JPO&INPIT