Method of calculating height of curved surface of microscope slide glass and microscope

摘要

The invention relates to the field of microscope imaging technology, in particular to calculate the height of a glass slide and estimate the position of the glass slide in a microscope coordinate system by means of height values, under continuous scanning in the case of high magnification magnification. It relates to a method of acquiring a clear image. In the present invention, by using a standard slide glass as a reference point, height values of all standard measurement points on another measurement target slide glass are obtained. The said measurement method is acquired by the conversion relation of a function simultaneous equation of a position coordinate and height value. According to the present invention, the height of the curved surface of any one slide glass can be accurately calculated, and the method of measuring the height of the curved surface can be used for an automatic scanning microscope. This provides a fast and micro scan of the image relative to the sample object on the slide glass. In particular, in the case of high magnification magnification lenses, the advantages of the invention of high imaging definition, high scanning speed, high efficiency and automation are more pronounced.