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Juvenile hormone sensor

机译:少年激素传感器

摘要

PROBLEM TO BE SOLVED: To develop and provide a juvenile hormone sensor capable of detecting and quantifying juvenile hormones at high sensitivity.SOLUTION: A juvenile hormone-specific biosensor protein is provided, which is a juvenile hormone-binding protein of lepidopteran origin modified or linked, at a specific side, with two types of phosphors having different fluorescence characteristics, or the like. When a juvenile hormone binds with the sensor protein, a conformational change of the juvenile hormone-binding protein causes a resonance energy transfer (FRET) between the phosphors. High-sensitivity detection and quantification of the juvenile hormone can be done by detecting the change in energy.SELECTED DRAWING: None
机译:解决的问题:开发和提供一种能够高灵敏度地检测和定量少年激素的少年激素传感器。解决方案:提供了一种少年激素特异性生物传感器蛋白,该蛋白是鳞翅目起源的少年激素结合蛋白,经过修饰或连接。在特定的一面,用两种具有不同荧光特性的磷光体等。当少年激素与传感器蛋白结合时,少年激素结合蛋白的构象变化会导致荧光粉之间发生共振能量转移(FRET)。可以通过检测能量变化来进行幼激素的高灵敏度检测和定量。

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