METHOD FOR PRODUCING ENZYME DERIVED FROM PSYCHROPHILIC OR MESOPHILIC MICROORGANISM

摘要

A method for producing an enzyme derived from a psychrophilic or mesophilic microorganism, said method comprising: a first step for introducing, into a thermophilic microorganism having an optimum growth temperature of 50^oC or higher, a DNA encoding an enzyme derived from at least one kind of microorganism selected from among a psychrophilic microorganism and a mesophilic microorganism, the optimum growth temperature of which is 50^oC or lower and lower by at least 10^oC than the optimum growth temperature of the thermophilic microorganism,to thereby obtain a transformant; a second step for culturing and growing the transformant at a culture temperature of 50^oC or higher; and a third step for, after the second step, changing the culture temperature to 50^oC or lower and lower by at least 10^oC than the culture temperature in the second step to thereby express the enzyme.