RECOMBINANT VECTOR COMPRISING CYTOCROME P450 REDUCTASE GENES, MICROORGANISM TRANSFORMED THEREOF AND METHOD FOR PRODUCING P450 ENZYME-DERIVED COMPOUNDS USING THE SAME

摘要

The present invention is a recombinant gene comprising a CPR to increase the catalytic activity of the P450 enzyme vector, the recombinant vector and P450 relates to a process for the production of transformed bacteria and P450 catalytic reaction using the same compound by a recombinant vector containing the gene. A recombinant vector according to the present invention, to increase the catalytic activity of the P450 enzyme, the rice plant origin composed of a nucleotide sequence of SEQ ID NO: 12 NADPH- cytochrome P450 reductase 2 (NADPH-cytochrome P450 reductase2) A recombinant vector containing the gene includes. The transformed bacteria according to the invention also, NADPH- Cytochrome consisting of rice-derived nucleotide sequence of SEQ ID NO: 12 P450 reductase 2 (NADPH-cytochrome P450 reductase2) with a recombinant vector containing the gene; Includes a 5-hydroxy-tryptamine is dehydratase (tryptamine 5-hydroxylase) or a recombinant vector comprising a gene mate scenario is 4-hydroxy-dehydratase (cinnamate 4-hydroxylase) bacteria transformed by the recombinant vector containing the gene . The trait can be reliably produce large amounts of serotonin or via a switch Kumar acid bacteria. ;