METHOD FOR PROMOTING REPLICATION OF BOVINE RHINOTRACHEITIS VIRUSES USING COLD ATMOSPHERIC PLASMA

摘要

A method for promoting replication of infectious bovine rhinotracheitis viruses using cold atmospheric plasma, including: irradiating a medium for Madin-Darby bovine kidney cells with a cold atmospheric plasma generator; adding the irradiated medium to the Madin-Darby bovine kidney cells; and adding infectious bovine rhinotracheitis viruses for incubation. The time required for treatment is 2 min allowing for a simple and rapid operation. The plasma is used for the indirect treatment of cells with a uniform process and a controllable intensity. The replication of the infectious bovine rhinotracheitis viruses in the Madin-Darby bovine kidney cells is significantly promoted by co-incubation in the treated DMEM for 1 hour, so that the high levels of infectious bovine rhinotracheitis viruses obtained can be used for vaccine production after inactivation, improving the vaccine production efficiency.