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A method of suppressing viral replication of infectious bovine rhinotracheitis in cell culture
A method of suppressing viral replication of infectious bovine rhinotracheitis in cell culture
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机译:在细胞培养中抑制传染性牛鼻气管炎病毒复制的方法
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摘要
1. method u043fu043eu0434u0430u0432u043bu0435u043du0438u00a0 reproduction virus infection u0440u0438u043du043eu0442u0440u0430u0445u0435u0438u0442u0430 cattle in culture cells with the help of antiviral drug, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 the antiviral drug ribavirin u0438u0441u043fu043eu043bu044cu0437u0443u0435u0442u0441u00a0.;2. method for u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 1, so that the culture of cells MDBK, u0432u044bu0440u0430u0449u0435u043du043du0443u044e u043cu0438u043au0440u043eu043cu0435u0442u043eu0434u043eu043c in u043au0443u043bu044cu0442u0443u0440u0430u043bu044cu043du044bu0445 96 hole u043fu043bu0430u043du0448u0435u0442u0430u0445, u0432u043du043eu0441u00a0u0442 virus in a dose of 1 - 10 u0442u0446u043450 / tc, u0438u043du043au0443u0431u0438u0440u0443u044eu0442 to are with the virus during the 1.5 - 2 hours, then u0432u043du043eu0441u00a0u0442 u043fu0440u043eu0442u0438u0432u043eu0432u0438u0440u0443u0441u043du044bu0439 drug ribavirin in a non-toxic dose of 501 mg / ml.;3. method for u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 1, so that the mixture u0438u043du043au0443u0431u0438u0440u0443u044eu0442 within 72 hours at a temperature of 370,2u0441 u0443u0441u043bu043eu0432u0438u00a0u0445 50.2% of co2 in the atmosphere, at the end of the incubation u0432u0438u0440u0443u0441u0441u043eu0434u0435u0440u0436u0430u0449u0443u044e su u0441u043fu0435u043du0437u0438u044e u0442u0438u0442u0440u0443u044eu0442 u0434u043bu00a0 u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 antiviral effect of this drug.
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