PARASITIC PHYTOPHTHORA-DERIVED OMEGA-3 FATTY ACID DESATURASE FOR SYNTHESIZING POLYUNSATURATED FATTY ACIDS, CARRIER CONTAINING FATTY ACID DESATURASE, RECOMBINANT MICROORGANISMS, AND APPLICATION THEREOF

摘要

It relates to w-3 fatty acid desaturase for the synthesis of pufas from phytophthora parasitica, a plasmid containing the same, a recombinant microorganism containing the plasmid and its application. The invention also provides the use of the above ω-3 fatty acid desaturase in the biosynthesis of PUFAs, especially to catalyze C20:4^{Δ5,8,11,14} to C20:5^{Δ5,8,11,14,17} at normal temperature, with catalytic efficiency 65%. The recombinant strain reached 31.5% of the total fatty acid, and the conversion rate to AA was up to 77.6%,