A recombinant nucleic acid sequence, as shown in SEQ ID NO.3, of parasitic phytophthora-encoded ω-3 fatty acid desaturase, a carrier containing ω-3 fatty acid desaturase, recombinant saccharomyces cerevisiae and recombinant mortierella alpina which contain the carrier, and a construction method of a recombinant engineering strain. According to the use of the recombinant saccharomyces cerevisiae in the biosynthesis of polyunsaturated fatty acids, the recombinant saccharomyces cerevisiae can catalyze C20:4 Δ5,8,11,14 into C20:5 Δ5,8,11,14,17 at room temperature, and the catalytic efficiency reaches 65%. The content of EPA in a fermentation product of the recombinant mortierella alpina reaches 31.5% of the total amount of fatty acids.
展开▼
机译：SEQ ID NO.3所示的重组疫霉菌编码的ω-3脂肪酸去饱和酶，含有ω-3脂肪酸去饱和酶的载体，酿酒酵母和含有该载体的重组高山被孢霉的重组核酸序列，以及一种重组工程菌的构建方法。根据重组酿酒酵母在多不饱和脂肪酸的生物合成中的用途，该重组酿酒酵母在室温下可以将C20：4Δ5,8,11,14催化为C20：5Δ5,8,11,14,17，催化效率达到65％。重组高山被孢霉菌发酵产物中的EPA含量达到脂肪酸总量的31.5％。
展开▼
