method of modifying target nucleic acid molecules in cells, molecule, recombinant funnel molecule, vector and its use, vector system, target cell modification system in cells, composition

摘要

target nucleic acid modification methods. The method involves a construct in which a guide is covalently linked to a donor (fusion) to be introduced into the target nucleic acid by homologous recombination and is based on introducing nuclease, such as crispr or talen, into the cell containing the target nucleic acid. The fusion node may be introduced as a dna vector.