bacterial spore germination method; and nutrient germination composition.

摘要

a nutrient germination composition to aid in spore germination and a method for increasing spore germination efficiency. The composition comprises 1-amino acids, d-glucose and / or d-fructose, a phosphate buffer, an industrial preservative, and may include bacterial spores or they may be combined separately for germination. The method comprises providing a germinal composition of bacterial nutrients and spores, preferably from one or more bacillus species, and heating to a preferred elevated temperature between 41 ° C and 44 ° C for an incubation period of about 2 to 60 minutes. The germinal nutrient composition is preferably in a concentrated liquid form which is diluted immediately prior to initiating the point of use germination / incubation method. The method may also include distributing a germinated spore solution to a point of use / consumption, such as animal feed, water or litter, or a wastewater or drainage system.