PROCESS FOR IDENTIFYING AMYLOID PRECURSOR PROTEIN IN PROTEIN COMPLEXES OF HIGH MOLECULAR WEIGHT

摘要

The invention relates to a process for identifying native amyloid precursor protein in protein complexes of high molecular weight, isolated from cellular membranes, meant to be applied in Alzheimer's disease pathology. According to the invention, the process consists in separating the cell membranes from the other cell components, extracting the protein complexes with 0.5...1% detergent Triton-X 100 in 50...75 mM of imidazole buffer, followed by the separation of protein complexes by electrophoresis in polyacrylamide as gel, for 2...4 h, at constant amperage of 10mA/gel on ice, in electrophoresis buffer with a pH 8...10 and the complex transfer on transfer membrane, for 20...22 h, at constant amperage of 100 mA, on ice, followed by protein identification with specific antibodies.