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Use of enzymes that catalyze the synthesis of pyruvate from formate and acetyl-CoA and bacteria that express the same
Use of enzymes that catalyze the synthesis of pyruvate from formate and acetyl-CoA and bacteria that express the same
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机译:使用能催化甲酸和乙酰辅酶A合成丙酮酸的酶以及表达相同酶的细菌
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摘要
An isolated microorganism that is genetically modified to express pyruvate formate lyase (PFL) or 2-ketobutyrate formate lyase, where the gene that codes for isocitrate lyase (aceA) and / or malate synthase is deleted in the microorganism, where acetyl-CoA of the microorganism is converted to pyruvate in the presence of formate in a single-stage reaction, where the net flow of the reaction is in the direction of pyruvate synthesis.
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