DYNAMIC LIGHT SCATTERING NANOPLATFORM FOR HIGH-THROUGHPUT TRANSCRIPTION FACTOR-DNA INTERACTION DETERMINATION FOR DRUG SCREENING

摘要

Methods, systems and nanoprobes for identifying sequence-specific transcription factor DNA interactions for drug screening are provided. The method includes homogeneously mixing plasmonic metal nanoparticle probes with protein samples activated or reactivated by a drug as an assay in multi-well plates, measuring particle size distribution of the assembly of the plasmonic metal nanoparticle probes in the solution by dynamic light scattering and determining one or more sequence-specific transcription factor DNA interactions from a curve of the particle size distribution. The plasmonic metal nanoparticle probes comprise a plurality of plasmonic metal nanoparticles and a transcription factor-specific response element and the protein samples bind with the specific response elements to form an assembly of the plasmonic metal nanoparticle probes. The specific response element comprises a specific transcription factor response element including a transcription factor selected from p53, p73, NF-ĸΒ, FOXP3 and signal transducers and activators of transcription (STAT) family.