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Dynamic Light Scattering Nanoplatform For High-Throughput Drug Screening

机译:动态光散射纳米平台用于高通量药物筛选

摘要

Methods, systems and nanoprobes for identifying sequence-specific transcription factor DNA interactions for drug and/or disease screening are provided. The method includes homogeneously mixing plasmonic metal nanoparticle probes with protein samples as an assay in multi-well plates. The plasmonic metal nanoparticle probes comprise a plurality of plasmonic metal nanoparticles and a specific DNA response element and the protein samples bind with the specific response elements to form an assembly of the plasmonic metal nanoparticle probes. The method further includes measuring particle size distribution of the assembly of the plasmonic metal nanoparticle probes in the solution by dynamic light scattering and determining one or more sequence-specific transcription factor DNA interactions from a curve of the particle size distribution determined from light scattered by the dynamic light scattering. The nanoprobe includes a plurality of plasmonic metal nanoparticles and a DNA linker. The DNA linker forms a link between the two plasmonic metal nanoparticles, the DNA linker including a double stranded region encoding a specific response element.
机译:提供了用于鉴定用于药物和/或疾病筛选的序列特异性转录因子DNA相互作用的方法,系统和纳米探针。该方法包括将等离激元金属纳米颗粒探针与蛋白质样品均匀混合,作为在多孔板中的测定。等离子体金属纳米颗粒探针包括多个等离子体金属纳米颗粒和特异性DNA反应元件,并且蛋白质样品与特异性反应元件结合以形成等离子体金属纳米颗粒探针的组装体。该方法进一步包括通过动态光散射来测量溶液中的等离子体金属纳米颗粒探针的组件的粒径分布,并根据由由纳米粒子散射的光确定的粒径分布曲线确定一种或多种序列特异性转录因子DNA相互作用。动态光散射。纳米探针包括多个等离激元金属纳米颗粒和DNA接头。 DNA接头形成两个等离激元金属纳米颗粒之间的链接,DNA接头包括编码特定反应元件的双链区。

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