METHODS AND KITS OF CONSTRUCTING SEQUENCING LIBRARY FOR USE IN DETECTING CHROMOSOME COPY NUMBER VARIATION

摘要

Provided herein is a method of constructing a high throughput sequencing library for use in detecting chromosome copy number variation comprising mainly the following steps: (1) subjecting DNAs to be tested to random fragmentation by a double-strand DNA fragmentation enzyme; (2) end-filling and adding poly-adenine at the 3'end of the fragmented DNAs; (3) connecting the end-filled DNAs having a 3'end poly-adenine with sequencing linkers to obtain connected products; (4) purifying the connected products to obtain the sequencing library; wherein steps (1) - (3) are performed in a single reaction tube.Also provided is a kit for constructing a sequencing library for use in detecting chromosome copy number variation.