Methods and kits for constructing sequencing libraries for use in the detection of chromosomal copy number variation

摘要

A method of constructing a high-throughput sequencing library for use in the detection of chromosomal copy number variation, the following steps: (1) Random fragmentation of the DNA under test with double-stranded DNA fragmentation enzyme. Steps to apply, (2) end-smoothing the 3'end of the fragmented DNA and adding polyadenine, (3) ligating the end-smoothing DNA having 3'end polyadenine with a linker to obtain a ligated product. (4) A method comprising mainly purifying the linked product to obtain the sequencing library, wherein steps (1) to (3) are performed in a single reaction tube. Provided herein. Kits for building sequencing libraries for use in detecting chromosomal copy number variation are also provided.