A New Detecting Method for the Sequence Difference of CHDI Gene on Sex Chromosomes Z and W in Domesticated Ducks of China

摘要

A new method for detecting the sequence differences of duck Chromo-helicase-DNA-Binding 1( CHD1) genes on sex chromosomes Z and W was established by amplifying DNA fragment length poly morphism,which aimed to solve some technical problems,such as the sex identification with nondamage,the misclassifc rate of artificial sex identification during embryonic period,and the injury caused by anal reversal identification method during neonatal period. The improved sex identification method was developed by DNA amplified fragment length polymorphism by using CHD1 gene sequence in bird. The sex identification PCR primers YPF/YPR were used,and the PCR product was cloned,sequenced and blasted; and the effectiveness and accuracy of this method were verified through living examples. 2% agarose gel electrophoresis was used to distinguish 495 bp CHD1-Z and 351 bp CHD1-W obtained by primer YPF/YPR. The female( ZW) displayed two visible bands; while male( ZZ) had only one single band. It was proved that this method for detecting the sequence differences of duck CHD1 gene was visual and reliable,and the molecular marker provided was effective and precise in the sex molecular biological identification of domesticated duck in China.