METHOD FOR ESTABLISHING SERUM GLYCOPROTEIN GLYCOME PROFILE MODEL OF LIVER FAILURE

摘要

Disclosed is a method for establishing a serum glycoprotein glycome profile model of liver failure. The method uses a G-Test detection method to detect a serum glycoprotein glycome profile, establishes a glycome profile model, with liver failure patients and normal controls having a significant difference therebetween, and screens for NA2, the expression thereof being significantly different between the liver failure group and the normal control group. Based on the constructed N-glycome profile model, the detection sensitivity and specificity for liver failure reach 100.0% and 97.6%, respectively. In subsequent applications, the degree of liver failure of persons to be tested can be detected by comparing the peak value of the singlet NA2 in the N-glycome profile of the serum of the persons to be tested with that thereof in the established profile model. The method can enable many patients suffering from liver failure to undergo routine, non-invasive detection, helps doctors and patients to monitor the occurrence and disease progression of liver failure in a timely manner, and is expected to be generalized and used clinically.