METHOD FOR CONSTRUCTING HIGH-EXPRESSION VECTOR OF HUMAN ARNTL GENE, AND APPLICATIONS

摘要

Provided are a method for constructing a high-expression vector of a human ARNTL gene, and applications. The method comprises the steps of: firstly, amplifying and purifying a target gene : designing an upper primer and a lower primer, extracting a cell RNA, and carrying out PCR amplification by using a reversely-transcribed cDNA as a template, and after a target band is cut, carrying out recycling and purification by using a reagent kit; and secondly, constructing and verifying a recombinant plasmid pCDNA3.1-ARNTL: after double enzyme digestion is carried out on the purified PCR product and a vector pCDNA3.1 by using Xba I and EcoR I, respectively recycling and purifying the enzyme digestion products by using reagent kits, connecting segments, carrying out screening, culture, cloning and sequencing, and detecting the gene expression condition by using a QPCR.