RAPID DETECTION METHOD FOR SALMONELLA IN DAIRY PRODUCT

摘要

A rapid detection method for Salmonella in a dairy product, mainly comprising the steps of pretreating the dairy product, extracting a DNA template in a sample to be tested, formulating a LAMP amplification reaction solution, and LAMP amplification and detection, where eosin and 2-hydroxy-3-naphthoic acid are added to the LAMP amplification reaction solution to allow a positive amplification product to express a specific fluorescent reaction, thus determining the presence of Salmonella. This detection method for Salmonella in the dairy product is simple, time-saving, highly accurate, and highly sensitive, requires merely 3-3.5 hours from the pretreatment of the dairy product to the acquisition of the final detection result, thus greatly increasing the freshness and shelf life of the dairy product being inspected; the positive coincidence rate of the result detected per the present method and C12Q of the result detected according to steps in GB 4789.4-2016 is 99.9% or more.