DETECTION METHOD OF SOMATIC GENETIC ANOMALIES, COMBINATION OF CAPTURE PROBES AND KIT OF DETECTION

摘要

This invention relates to a detection method of somatic genetic anomalies potentially present in a tissue sample cancerous, taken from a subject, wherein said detection method comprises the following steps: (a) shearing of the genomic DNA extracted from said tissue sample, to obtain sheared genomic DNA; (b) preparing a library by hybridization capture from the sheared genomic DNA obtained at step (a), by the implementation of at least 43 capture probes having at least 110 nucleotides in length and at least 75% sequence homology with respectively SEQ ID NO:1 to SEQ ID NO:43, said capture probes being able to hybridize on fragments from at least 5 target regions selected from : ALK, BRAF, EGFR, EIF1AY, ERBB2, ERBB4, KDR, KIT, KRAS, MAP2K1, MET, NRAS, PDGFRA, SLC28A1, TP53; (c) sequencing of sheared genomic DNA contained in the library issued at step (b), and (d) analyzing of the sequences of said sheared genomic DNA obtained at step (c), for detecting somatic genetic anomalies in said tissue sample. The present invention also refers to a combination of capture probes and a kit comprising said combination.