A polymer-substrate material having a covalently coupled, enzymatically degradable peptide sequence

摘要

The present invention relates to a polymer-based material having covalently bound and enzymatically degradable peptide sequences that are not degradable by biological activity and metabolic activity of cells and tissues, wherein each of the peptide sequences comprises 2 to 15 amino acids And incorporated into the polymer-based material or bonded onto the polymer-based material. Thus, the peptide sequence may be part of a two-dimensional or three-dimensional structure of the polymer-based material. By bio-orthogonal degradation of the three-dimensional structure is achieved by enzyme-controlled-degradation of covalent bonds in the peptide sequence, or when peptide sequences are conjugated on the polymer-based material, at least a portion Is achieved. The invention further relates to a method for the in vitro production of cell cultures or tissues or organs; For in-vivo stabilization of donated cells, tissues or organs; And to the use of such polymer-based materials for in vivo or in vitro treatment of living cells, tissues and organs. On the one hand, the adhesive bond between the material and the other sample, cell, tissue or organ does not destroy the integrity of the sample (extracellular matrix and cell-cell and extracellular matrix-cell junction) (Bio-orthogonality).