- A mutant of L-rhamnose isomerase from Clostridium stercorarium and A method for producing of D-allose from D-allulose using the same

摘要

The present invention relates to an L-rhamnose isomerase mutant from clostridium stercorarium and a method to produce allose (D-allose) from allulose (D-allulose) by using the same. When the L-rhamnose isomerase mutant of the present invention is used as a biocatalyst for a reaction producing allose from allulose, the present invention is able to maximize a production effect while greatly reducing production costs since allose conversion efficiency from allulose and productivity increase meaningfully compared to a wild type enzyme, and an improved yield can be represented while using a substrate having a low price compared to an allose production reaction by existing technology. Therefore, the mutant L-rhamnose isomerase of the present invention can be useful as a biocatalyst of an allose production process.