CRISPR- CRISPR-BASED GENOME MODIFICATION AND REGULATION

摘要

summary The present invention provides RNA-derived endonucleases, which are processed for expression in eukaryotic cells or embryos, and methods of using RNA-derived endonucleases for targeted genomic modifications in eukaryotic cells or embryos. To provide. Fusion proteins are also provided, wherein each fusion protein comprises a CRISPR / Cas-like protein or fragment and effector domain thereof. The effector domain may be a cleavage domain, epigenetic modification domain, transcriptional activation domain, or transcriptional repressor domain. Methods are also provided for altering chromosomal sequences or controlling the expression of chromosomal sequences using fusion proteins.