CRISPR- CRISPR-BASED GENOME MODIFICATION AND REGULATION

摘要

summary The present invention provides RNA-derived endonucleases, which are processed for expression in eukaryotic cells or embryos, and methods of using RNA-derived endonucleases for targeted genome modification in eukaryotic cells or embryos. Provides. Fusion proteins are also provided, wherein each fusion protein comprises a CRISPR/Cas-like protein or fragment thereof and an effector domain. The effector domain can be a cleavage domain, an epigenetic modification domain, a transcription activation domain, or a transcription repressor domain. Methods for altering chromosomal sequences or controlling expression of chromosomal sequences using fusion proteins are also provided.