New ketol-acid reductoisomerase with a cofactor preference to NADH developed

摘要

The present invention relates to an enzymatic modification of ketol-acid reductoisomerase essential for the production of isobutanol. More specifically, the enzyme was modified through the Rossman loop remodeling to make Escherichia coli-derived ketol-acid reductoisomerase having affinity for NADPH have affinity for NADH.