FRET White Colored Emitting Graphene Quantum Dots Composition for FRET Mediated Biosensing And Manufacture Method Thereof

摘要

The present invention relates to a white-emitting graphene quantum dot composition for FRET-mediated biosensing and a method of making the same. The white emitting graphene quantum dot of the present invention is a quantum dot having a graphene structure and exhibiting excellent optical controllability, low cytotoxicity, and excellent optical stability, which is produced by a thermal decomposition method. The manufacturing cost is low and the manufacturing is easy. In addition, the white light-emitting graphene dot and the manganese oxide-coated white light-emitting graphene quantum dot of the present invention have a negative charge surface charge and an average particle diameter of 20 nm, which is excellent in biostability and excellent in cell membrane permeability. The white light-emitting graphene quantum dot coated with manganese oxide of the present invention is coated with manganese oxide reduced by hydrogen peroxide or glutathione and gives a FRET-mediated fluorescence quenching effect, so that hydrogen peroxide or glutathione present in a biological sample is detected or a concentration value is calculated There is an effect that can be done. The manganese oxide has the advantage of being selectively reduced to hydrogen peroxide or glutathione present in the biological sample because the manganese oxide has a specificity of being reduced only by hydrogen peroxide or glutathione without being reduced by protein, polysaccharide, amino acid, or the like present in the living body. The composition containing the white manganese oxide-coated white light emitting graphene quantum dot of the present invention is low cost and has excellent selectivity in comparison with the method of detecting hydrogen peroxide or glutathione by a conventional label. Therefore, the next generation hydrogen peroxide or It is expected to be used as a glutathione detection composition.