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Detection method of monoclonal antibody using mass spectrometry
Detection method of monoclonal antibody using mass spectrometry
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机译:质谱检测单克隆抗体的方法
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摘要
Provided is a method for locally selectively digesting a variable region of a Fab domain of a monoclonal antibody while suppressing the digestion of an Fc domain to more easily detect and quantify the protein. A porous body in which a monoclonal antibody is immobilized in pores and nanoparticles immobilized with a protease are contacted with each other in a liquid to perform selective protease digestion of the monoclonal antibody and the obtained peptide fragment is subjected to liquid chromatography mass spectrometry (LC-MS) Wherein a peptide having an amino acid sequence comprising an amino acid derived from the CDR2 region of the heavy or light chain of the monoclonal antibody is detected.
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