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Medium composition with improved sensitivity and selectivity for detecting Campylobacter and detecting method using thereof

机译:用于检测弯曲杆菌的灵敏度和选择性提高的培养基组合物及其检测方法

摘要

The present invention relates to a medium composition for detecting Campylobacter with improved sensitivity and selectivity, and a Campylobacter detection method using the same. More specifically, the present invention relates to a medium composition for detecting Campylobacter, comprising avibactam, and a Campylobacter detection method using the same. The Campylobacter detection method comprises the steps of: a) enriching and culturing a poultry rinsate; and b) streaking a solid medium prepared with the composition with the enriched culture fluid prepared in the step a) and culturing the same. The medium composition for detecting Campylobacter of the present invention, by containing avibactam, suppresses competing bacteria producing ESBL enzymes and thus can provide a medium composition for detecting Campylobacter having improved sensitivity and selectivity, a Campylobacter detection kit comprising the same, and a Campylobacter detection method using the composition. In particular, the avibactam of the present invention, since it can be contained in a smaller amount than other antibiotics included in the detection medium, is less likely to cause antibiotic resistance and thus can be extremely useful as a composition for detection.
机译:本发明涉及具有提高的灵敏度和选择性的用于检测弯曲杆菌的培养基组合物,以及使用该培养基组合物的弯曲杆菌检测方法。更具体地,本发明涉及一种包含avibactam的用于检测弯曲杆菌的培养基组合物以及使用该培养基组合物的弯曲杆菌的检测方法。弯曲杆菌的检测方法包括以下步骤:a)富集和培养家禽冲洗液; b)用步骤a)中制备的富集的培养液对用该组合物制备的固体培养基进行划线培养。通过含有阿维巴坦,本发明的用于检测弯曲杆菌的培养基组合物抑制产生ESBL酶的竞争细菌,因此可以提供具有提高的灵敏度和选择性的用于检测弯曲杆菌的培养基组合物,包括该培养基组合物的弯曲杆菌检测试剂盒以及弯曲杆菌检测方法。使用组成。特别地,由于本发明的avibactam的含量可以比检测介质中包含的其他抗生素的含量少,因此不易引起抗生素抗性,因此作为检测用组合物非常有用。

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