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Broth composition with improved sensitivity and selectivity for selectively enriching Campylobacter and detecting method using thereof

机译:用于选择性富集弯曲杆菌的灵敏度和选择性提高的肉汤组合物及其检测方法

摘要

The present invention relates to a selective enrichment medium composition for Campylobacter, comprising avibactam; and a Campylobacter detection method using the same. More specifically, the present invention relates to a Campylobacter detection method comprising: a) enriching and culturing a poultry rinsate in a medium prepared with the composition; and b) streaking a solid medium for Campylobacter detection with the enriched culture fluid prepared in the step a) and culturing the same. The selective enrichment medium composition for Campylobacter of the present invention, by containing avibactam, suppresses competing bacteria producing ESBL enzymes and thus can provide a selective enrichment medium composition for Campylobacter having improved sensitivity and selectivity, a Campylobacter detection kit comprising the same, and a Campylobacter detection method using the composition. In particular, the avibactam of the present invention, since it can be contained in a smaller amount than other antibiotics included in the selective enrichment medium, is less likely to cause antibiotic resistance and thus can be extremely useful as a composition for selective enrichment.
机译:本发明涉及弯曲杆菌的选择性富集培养基组合物,其包含阿维巴坦;和以及使用弯曲杆菌检测方法。更具体地,本发明涉及弯曲杆菌检测方法,其包括:a)在用所述组合物制备的培养基中富集和培养家禽漂洗液; b)用步骤a)中制备的富集培养液划线培养弯曲杆菌的固体培养基,并进行培养。通过含有阿维巴坦,本发明的用于弯曲杆菌的选择性富集培养基组合物抑制了产生ESBL酶的竞争细菌,因此可以提供具有提高的灵敏度和选择性的用于弯曲杆菌的选择性富集培养基组合物,包括该组合物的弯曲杆菌检测试剂盒以及弯曲杆菌检测方法使用组成。特别地,由于本发明的avibactam的含量比选择性富集培养基中包含的其他抗生素的含量少,因此不易引起抗生素抗性,因此作为选择性富集的组合物非常有用。

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