The present application discloses a method of manufacturing an organic light emitting diode display substrate having a subpixel region and a subpixel-subregion. The method includes forming a pixel confinement layer on a base substrate, the pixel confinement layer being formed in an inter-subpixel region and defining a subpixel region of the organic light emitting diode display substrate; Forming an insulating dielectric layer on a side of the pixel defining layer remote from the base substrate, wherein the insulating dielectric layer is formed to define a first opening region larger than the subpixel region; And subsequent to forming the insulating dielectric layer, forming an organic light emitting layer in each subpixel region using a mask plate disposed on the insulating dielectric layer.
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