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MULTIGENE EXPRESSION IN MICROALGAE

机译:微藻中的多基因表达

摘要

The present application relates to an expression system for multigene overexpression in microalgae, which expression system comprises at least two nucleic acid expression cassettes, wherein each expression cassette comprises a promoter operably linked to three or more transgenes connected to one another by at least one sequence encoding a 2A peptide (i.e. a multicistronic construct). Also disclosed herein are vector systems comprising said expression systems, host cells transformed with said expression systems or comprising said vector systems, methods for producing these host cells, as well as their use for biosynthesis.
机译:本申请涉及一种用于微藻中多基因过表达的表达系统,该表达系统包含至少两个核酸表达盒,其中每个表达盒包含与通过至少一个序列编码彼此相连的三个或多个转基因可操作地连接的启动子。 2A肽(即多顺反子构建体)。本文还公开了包含所述表达系统的载体系统,用所述表达系统转化的宿主细胞或包含所述载体系统的载体,产生这些宿主细胞的方法及其在生物合成中的用途。

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