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METHOD FOR QUANTIFYING AMINO ACID AND AMINO ACID QUANTIFICATION KIT

机译:氨基酸定量方法和氨基酸定量试剂盒

摘要

[Problem] To provide: a method for selectively and easily quantifying an amino acid to be measured (L-form or D-form amino acid) with high sensitivity using aminoacyl-tRNA synthetase (AARS); and an amino acid quantification kit. [Solution] The present invention pertains to: a method for quantifying an amino acid (L-form or D-form amino acid) in a sample by means of AARS, said method being characterized in that AARS and an amino acid are released from an aminoacyl-AMP AARS complex once formed and are used once again for forming an aminoacyl-AMP AARS complex so as to ultimately produce a reaction product such as pyrophoric acid to be measured in an amount that is greater in terms of the number of moles than the amino acid included in the sample; and an amino acid quantification kit for implementing said method.
机译:[问题]提供:一种使用氨基酰基-tRNA合成酶(AARS)以高灵敏度选择性且容易地定量被测定氨基酸(L型或D型氨基酸)的方法。和氨基酸定量试剂盒。 [解决方案]本发明涉及:一种通过AARS定量样品中氨基酸(L-形式或D-形式氨基酸)的方法,所述方法的特征在于AARS和一种氨基酸从一种氨基酸中释放出来。氨基酰基-AMP AARS络合物一旦形成,并再次用于形成氨基酰基-AMP AARS络合物,从而最终产生反应产物(如焦pyr酸),所测量的量以摩尔数计大于样品中包含的氨基酸;和用于实施所述方法的氨基酸定量试剂盒。

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