COMPREHENSIVE AND QUANTITATIVE LIPID AND TOCOPHEROL ANALYSIS

摘要

A method for determining in a sample, by mass spectrometry, the presence, absence or amount of one or more lipid species from one or more lipid classes is described herein. The one or more lipid classes are selected from the group consisting of wax esters (WE), squalene (SQ), triacylglycerols (TAG), diacylglycerols (DAG), free fatty acids (FFA), cholesteryl esters (CE), cholesterol (CH), and combinations thereof. The method includes a) subjecting the sample to an ionization source to produce one or more ions detectable by mass spectrometry from each of the one or more lipid species; b) measuring, by mass spectrometry, the amount of the one or more ions from each of the one or more lipid species; and c) using the measured amount of the one or more ions to determine the presence, absence or amount of each of the one or more lipid species.