Method of causing 19F chemical shift change by double strand formation

摘要

PROBLEM TO BE SOLVED: To provide a highly sensitive detection means suitable for in-vivo measurement for detecting double strand formation. SOLUTION: By using the fluorine-containing artificial nucleoside represented by the formula I, a modified nucleic acid having the fluorine-containing artificial nucleoside represented by the formula I introduced into a base sequence, and double-stranded formation with the modified nucleic acid are possible. A double-strand is formed between the nucleic acid and the nucleic acid, and a change in 19F chemical shift that can be detected by the nuclear magnetic resonance method in the comparison before and after the double-strand formation is caused. [Selection diagram] None