Method for producing a 19F chemical shift change by photocrosslinking

摘要

Provided is a highly sensitive detection means, suited to measurement in vivo, for detecting photocrosslinking formed by photoresponsive bases by using a method for causing a change in the 19F chemical shift detectable by nuclear magnetic resonance spectroscopy in a comparison of before and after photocrosslinking formation, the change being caused by forming photocrosslinking between a photoresponsive base having a photocrosslinkable vinyl structure and a photocrosslinkable fluorine-containing modified pyrimidine base by using a fluorine-containing modified pyrimidine base in which position 5 of the pyrimidine rings of thymine (T) or uracil (U) bases, which are photocrosslinkable to a photoresponsive base having a photocrosslinkable vinyl structure, has been substituted by group R (where group R is a fluorine atom or a C1-C4 fluoroalkyl group in which one or more hydrogen atoms have been substituted by fluorine atoms).