Mutant enzyme and method for producing terpenoid using the mutant enzyme

摘要

The object of the present invention is to efficiently produce useful terpenoid compounds, and specifically, to provide a method for preparing squalene, which is an important intermediate of terpenoid. The object can be solved by a hydroxymethylglutaryl CoAreductase (HMGR) comprising:(a) an amino acid other than alanine (A) at the 10th position in an S±2 amino acid sequence of HMGR, (b) an amino acid other than proline (P) at the 1 st position from the carboxyl terminal in the DKK region of the HMG-CoA binding site of HMGR, (c) an amino acid other than alanine (A) at the 1st position in an L±2 amino acid sequence of HMGR, and (d) an amino acid other than glutamic acid (E) at the 6th position in an L±2 amino acid sequence of HMGR of the present invention.